학술논문
Giant panda (Ailuropoda melanoleuca) sperm morphometry and function after repeated freezing and thawing.
Document Type
Article
Author
Source
Subject
*GIANT panda
*MORPHOMETRICS
*MAMMAL spermatozoa
*THAWING
*CRYOPRESERVATION of organs, tissues, etc.
*DENSITY gradient centrifugation
*HEALTH
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Language
ISSN
0303-4569
Abstract
This work examines the effects of subsequent cycles of freezing-thawing on giant panda (Ailuropoda melanoleuca) sperm morphometry and function, and assesses whether density-gradient centrifugation (DGC) can increase the number of freezing-thawing cycles this sperm can withstand. A sperm sample was collected by electroejaculation from a mature giant panda and subjected to five freezing-thawing cycles. Although repeated freezing-thawing negatively affected (P < 0.05) sperm motility and membrane integrity, in both nonselected and DCG-selected sperm samples, >60% of the sperm cells in both treatments showed acrosome integrity even after the fifth freezing cycle. In fresh semen, the sperm head length was 4.7 μm, the head width 3.6 μm, area 14.3 μm² and perimeter length 14.1 μm. The present results suggest that giant panda sperm trends to be resistant to repeated freezing-thawing, even without DGC selection. [ABSTRACT FROM AUTHOR]