부산대학교 도서관

Abstract The biochemical mechanisms involved in phagocytosis and the intracellular survival of Aeromonas hydrophila (Ah) in host macrophages (MΦs) are complex processes that affect infection success or failure. Thus, in the present study, we described the in vitro infection of Nile tilapia MΦs by a homologous bacterium and tested the effects of anti- A. hydrophila immunoglobulin Y (IgY) on the phagolysosomal activity and intracellular survival of the pathogen. The anti- Ah IgY modulated lysosomal acid phosphatase (LAP) activity as well as the production of reactive oxygen intermediates (ROIs) and nitric oxide (NO), thereby potentiating phagocytosis and the elimination of Ah. Thus, we assume that the specific IgY had a beneficial effect on infection control and postulated the use of the Nile tilapia MΦs as an important in vitro experimental model for the functional and therapeutic study of Ah infection. Highlights • The inoculation with MPs of Ah in the swim bladder of tilapia allowed the harvesting of activated macrophages. • Culture of the tilapia macrophages allowed the study of the phagocytic process during infection by Ah. • The IgY specificity for proteins from Ah neutralized the adhesion molecules and possible endo/exotoxins. • IgY modulated phagolysosomal activity, reducing the intracellular survival of Ah in the MΦs controlling the infection. [ABSTRACT FROM AUTHOR]