부산대학교 도서관

Proteins have a critical role in life, and their separation and detection are of high importance. In the present study, magnetic hydroxyapatite was reinforced with zeolite to become a novel adsorbent synthesized by microwave-assisted method to separate protein via magnetic solid-phase extraction. The synthesized procedure was optimized through the mass ratio of zeolite to hydroxyapatite and radiation time. The synthesized nanocomposite was characterized by techniques such as XRD, EDAX, FESEM, TEM, FTIR, VSM, and BET. Structural evaluation reveals that the magnetic zeolite as a core provides a bed for HAP spherical crystals formation and HAP crystals dispersed over the magnetic zeolites or as large grains located around it. The experimental data revealed the maximum separation was achieved at contact time 60 min, initial pH 2, protein concentration 1.2 mg/mL, and adsorbent dosage 50 mg without any salt in the solution. The maximum adsorption capacity was 82.62 mg/g. Desorption efficiency of 95% at initial pH 12 and separation efficiency more than 50% after 4 successive regeneration cycles are the advantages of the adsorbent. The adsorption studies showed that Freundlich and pseudo-second-order models were well fitted with the obtained experimental data. [ABSTRACT FROM AUTHOR]