학술논문
Development and Validation of an In-House Real-Time Reverse-Transcriptase Polymerase Chain Reaction Assay for SARS-CoV-2 Omicron Lineage Subtyping between BA.1 and BA.2.
Document Type
Article
Author
Source
Subject
*POLYMERASE chain reaction
*SARS-CoV-2 Omicron variant
*REVERSE transcriptase polymerase chain reaction
*SARS-CoV-2
*WHOLE genome sequencing
*INFECTION control
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Language
ISSN
1999-4915
Abstract
In order to rapidly differentiate sublineages BA.1 and BA.2 of the SARS-CoV-2 variant of concern Omicron, we developed a real-time reverse-transcriptase polymerase chain reaction to target the discriminatory spike protein deletion at amino acid position 69–70 (S:del69–70). Compared to the gold standard of whole genome sequencing, the candidate assay was 100% sensitive and 99.4% specific. Sublineage typing by RT-PCR can provide a rapid, high throughput and cost-effective method to enhance surveillance as well as potentially guiding treatment and infection control decisions. [ABSTRACT FROM AUTHOR]