학술논문
Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells.
Document Type
Article
Author
Source
Subject
*CANCER cells
*SINGLE-stranded DNA
*IMMUNOGLOBULINS
*CELL survival
*URACIL
*MOLECULAR diagnosis
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Language
ISSN
1359-7345
Abstract
We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (∼90%) while maintaining cell viability (＞85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved. [ABSTRACT FROM AUTHOR]