부산대학교 도서관

Genetic background affects polyp development in the Multiple intestinal neoplasia (ApcMin) mouse model. The Modifier of Min 1 (Mom1) locus accounts for ~50% of the variation in polyp multiplicity. We generated reciprocal congenic lines, such that the recipient C57BL/6J (B6) strain carries a donor C3H/HeJ (C3H) Mom1 allele, and the recipient C3H strain carries a donor B6 Mom1 allele. Hybrid progeny from congenic females mated to B6 ApcMin/+ males were analyzed. A single C3H Mom1 locus on the B6 background reduced small intestinal polyp numbers by 50% and colon polyp incidence by 66% compared to their susceptible B6 Mom1S/S ApcMin/+ siblings. These findings show that the C3H genome contains a resistant Mom1R locus. The reciprocal congenic line, which carries the susceptible B6 Mom1S locus on the C3H background, reduced small intestinal polyp numbers by 80% and colon polyp incidence by 95% compared to B6 Mom1S/S ApcMin/+ mice. These data demonstrate that unidentified modifiers in the C3H strain can suppress intestinal polyp multiplicity in ApcMin/+ mice, and act in the absence of a resistant Mom1R locus. [Copyright &y& Elsevier]