학술논문
Total RNA Isolation From Picea mariana Dry Seed.
Document Type
Article
Source
Subject
*RNA
*CONIFER seed
*SPRUCE
*TISSUES
*CHLORIDES
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*
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Language
ISSN
0735-9640
Abstract
Isolating RNA from dry conifer seeds can be difficult because of a number of interfering compounds present in seeds. We describe a protocol for total RNA isolation from black spruce dry seeds, which is an adaptation of a method used for mouse myeloma tissues. The extraction relies on selective precipitation of RNA by using lithium chloride. [ABSTRACT FROM AUTHOR]