부산대학교 도서관

Vβ9Vδ2 T lymphocytes are the major human peripheral gd T cell subset, with broad reactivity against stressed human cells, including tumor cells. Vβ9Vδ2 T cells are specifically activated by small phosphorylated metabolites called phosphoantigens (PAg). Stress-induced changes in target cell PAg levels are specifically detected by butyrophilin (BTN)3A1, using its intracellular B30.2 domain. This leads to the activation of Vβ9Vδ2 T cells. In this study, we show that changes in the juxtamembrane domain of BTN3A1, but not its transmembrane domain, induce a markedly enhanced or reduced βδ T cell reactivity. There is thus a specific requirement for BTN3A1's juxtamembrane domain for correct βδ T cell-related function. This work identified, as being of particular importance, a juxtamembrane domain region of BTN3A molecules identified as a possible dimerization interface and that is located close to the start of the B30.2 domain. [ABSTRACT FROM AUTHOR]