학술논문
Ethylene-Induced Vinblastine Accumulation Is Related to Activated Expression of Downstream TIA Pathway Genes in Catharanthus roseus.
Document Type
Article
Source
Subject
*ALKALOIDS
*ANALYSIS of variance
*ETHYLENE
*FACTOR analysis
*GENE expression
*HIGH performance liquid chromatography
*HYDROGEN peroxide
*MEDICINAL plants
*OXIDATION-reduction reaction
*OXIDOREDUCTASES
*POLYMERASE chain reaction
*PROBABILITY theory
*RESEARCH funding
*RNA
*VINBLASTINE
*REVERSE transcriptase polymerase chain reaction
*DATA analysis software
*DESCRIPTIVE statistics
*PHARMACODYNAMICS
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Language
ISSN
2314-6133
Abstract
We selected different concentrations of ethephon, to stress C. roseus. We used qRT-PCR and HPLC followed by PCA to obtain comprehensive profiling of the vinblastine biosynthesis in response to ethephon. Based on our findings, the results showed that the high concentration of ethephon had a positive effect at both transcriptional and metabolite level. Meanwhile, there was a remarkable decrease of hydrogen peroxide content and a promoted peroxidase activity in leaves. The loading plot combination with correlation analysis suggested that CrPrx1 could be regarded as a positive regulator and interacts with ethylene response factor (ERF) to play a key role in vinblastine content and peroxidase (POD) activity. This study provides the foundation for a better understanding of the regulation and accumulation of vinblastine in response to ethephon. [ABSTRACT FROM AUTHOR]