부산대학교 도서관

To study morphology of Lactococcus by gram staining and to determine its shape and size. Novel Lactic acid bacteria isolated from the milk sources were checked for the gram staining property. Greezefree slides with a drop of liquid culture of selected isolate from milk sources were placed under laminar air flow for air drying and heat fixation. Upon which bacterial morphology was checked through gram staining and viewed under a compound microscope with different magnification levels. 2 groups with 20 samples per group were analysed by keeping threshold 0.05 and G power 80% coincidence interval 95% and enrolment ratio as 1. Selected isolates were found gram positive, rod shaped and purple in colour. They were all classified as Lactobacillus and Lactococcus. Variations of organisms were found significant (p<0.001) in raw milk compared to milk powder however no significant difference was recorded in bacterial shape and size analysis between the groups (p>0.05). The Lactococcus count was found higher than Lactobacillus in both the sources however milk powder was found a good source of Lactococcus than raw milk which was given significant bacterial population variation (p<0.001) compared to milk powder. Based on gram staining the isolates are Lactobacillus and Lactococcus with size ranging <1µm-2 µm. [ABSTRACT FROM AUTHOR]