학술논문
Robust 4C-seq data analysis to screen for regulatory DNA interactions.
Document Type
Article
Author
Source
Subject
*DNA
*DATA analysis
*GENETIC regulation
*COMPUTATIONAL biology
*GENE expression
*PROMOTERS (Genetics)
*CHROMOSOMES
*CONFORMATIONAL analysis
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Language
ISSN
1548-7091
Abstract
Regulatory DNA elements can control the expression of distant genes via physical interactions. Here we present a cost-effective methodology and computational analysis pipeline for robust characterization of the physical organization around selected promoters and other functional elements using chromosome conformation capture combined with high-throughput sequencing (4C-seq). Our approach can be multiplexed and routinely integrated with other functional genomics assays to facilitate physical characterization of gene regulation. [ABSTRACT FROM AUTHOR]