부산대학교 도서관

Objectives The purpose of this study was to determine whether rebamipide, an antistomach ulcer agent, ameliorated benzodiazepine-induced hyposalivation in rat parotid gland ( PG) and submandibular gland ( SMG). Methods Saliva was collected from PG and SMG through a capillary cannula inserted into the parotid duct and sublingual papillae, respectively, every 15 min for 1 h after stimulation with pilocarpine dissolved in physiological saline and intraperitoneally administered at 1 mg kg−1. Diazepam ( DZP) was administered intraperitoneally at a dose of 0.2 mg kg−1 twice daily for 7 days. Rebamipide was administered at 10, 20, 30, or 100 mg kg−1 concomitantly with DZP to determine its effect on hyposalivation. The effect of rebamipide on movement of intracellular calcium ([Ca2+] i) in isolated parotid acinar cells was analyzed using Fluo4, a fluorescent dye used to detect Ca2+. Results Repetitive administration of DZP decreased salivary secretion in PG and SMG. This inhibitory effect was weakened by administration of rebamipide. Prior administration of DZP (10−6M) significantly suppressed carbachol (10−7M)-induced increase in [Ca2+] i. This inhibitory effect was ameliorated by combined use with rebamipide (5 × 10−4M). Conclusion This findings suggest that rebamipide weakens the downregulatory effect of DZP on salivary secretion by preventing DZP-induced suppression of increase in [Ca2+] i. [ABSTRACT FROM AUTHOR]