학술논문
Experimental evidence of a pathogenic change caused by homologous recombination between endogenous and introduced dysfunctional Avr- Pita genes in Pyricularia oryzae.
Document Type
Article
Source
Subject
*PYRICULARIA oryzae
*RICE blast disease prevention
*GRAIN varieties
*MICROBIAL virulence
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Language
ISSN
1345-2630
Abstract
To provide experimental evidence that somatic homologous recombination (HR) is involved in the instability and diversification of the avirulence gene Avr- Pita in Pyricularia oryzae, we generated a dysfunctional Avr- Pita homolog and integrated it into strain Hoku-1 containing Avr- Pita. In the transformants, the occurrence of somatic HR events between Avr- Pita and the dysfunctional homolog was confirmed by PCR-RFLP. Germlings from conidia from the HR-positive transformants had lost the avirulence function, which enabled it to infect rice cultivar Yashiromochi containing the corresponding resistant gene Pi- ta. These results suggested that genetic mutation caused by somatic HR is one of the mechanisms responsible for virulence diversity. [ABSTRACT FROM AUTHOR]