학술논문
Sensorless adaptive optics implementation in widefield optical sectioning microscopy inside in vivo Drosophila brain.
Document Type
Article
Author
Source
Subject
*BRAIN imaging
*OPTICAL microscopes
*DROSOPHILA as laboratory animals
*ADAPTIVE optics
*FLUORESCENCE microscopy
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Language
ISSN
1083-3668
Abstract
We present an implementation of a sensorless adaptive optics loop in a widefield fluorescence microscope. This setup is designed to compensate for aberrations induced by the sample on both excitation and emission pathways. It allows fast optical sectioning inside a living Drosophila brain. We present a detailed characterization of the system performances. We prove that the gain brought to optical sectioning by realizing structured illumination microscopy with adaptive optics down to 50 µm deep inside living Drosophila brain. [ABSTRACT FROM AUTHOR]