학술논문
Frequencies of HNA-1, HNA-3, HNA-4, and HNA-5 in the Danish and Zambian populations determined using a novel TaqMan real time polymerase chain reaction method.
Document Type
Article
Source
Subject
*POLYMERASE chain reaction
*ZAMBIANS
*NEUTROPHILS
*CAUCASIAN race
*NUCLEOTIDE sequence
*GENE frequency
*BIOLOGICAL assay
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Language
ISSN
0001-2815
Abstract
In this study, we report a novel real time polymerase chain reaction (Q- PCR) method using TaqMan probes for human neutrophil antigens ( HNA)-1, -3, -4, and -5 genotyping. The method was validated in a Caucasian Danish population, a Zambian population, and in clinical samples using three different methods: an in-house polymerase chain reaction with sequence-specific primers ( PCR-SSP) method, a commercial available PCR-SSP kit and a novel Q- PCR method. We observed no discrepancy in the genotype frequencies determined by the PCR-SSP methods and the TaqMan assay in the populations studied. In tests of a family of Nigerian origin and in samples carrying the rare SLC44A2*1:2 genotype, different results were produced by the commercial PCR-SSP kit and the real-time TaqMan assay. The TaqMan-based genotyping method was rapid and reproducible, allowing high-throughput HNA-1, -3, -4, and -5 genotyping. [ABSTRACT FROM AUTHOR]