학술논문
Immunohistochemical characterisation of the immune landscape in primary uveal melanoma and liver metastases.
Document Type
Article
Author
Mariani, Pascale; Torossian, Nouritza; van Laere, Steven; Vermeulen, Peter; de Koning, Leanne; Roman-Roman, Sergio; Lantz, Olivier; Rodrigues, Manuel; Stern, Marc-Henri; Gardrat, Sophie; Lesage, Laetitia; Champenois, Gabriel; Nicolas, André; Matet, Alexandre; Cassoux, Nathalie; Servois, Vincent; Romano, Emanuela; Piperno-Neumann, Sophie; Lugassy, Claire; Barnhill, Raymond
Source
Subject
Language
ISSN
0007-0920
Abstract
Background: The immune landscape of uveal melanoma liver metastases (UMLM) has not been sufficiently studied. Methods: Immune cell infiltrates (ICIs), PD-1 and PD-L1 were characterised in 62 UMLM and 28 primary uveal melanomas (PUM). ICI, PD-1 and PD-L1 were scored as: (1) % tumoral area occupied by tumour-infiltrating lymphocytes or macrophages (TILs, TIMs) and (2) % perTumoral (perT) area. ICIs and other variables including histopathologic growth patterns (HGPs), replacement and desmoplastic, of UMLM were analysed for their prognostic value. Results: ICIs recognised by haematoxylin-eosin-saffron (HES) and IHC (e.g., T cells (CD3), B cells (CD20). Macrophages (CD68), (CD163), were primarily localised to the perT region in PUM and UMLM and were more conspicuous in UMLM. HES, CD3, CD4, FoxP3, CD8, CD20, PD-1 TILs were scant (<5%). TIMs were more frequent, particularly in UMLM than in PUM. Both CD68+ TIMs and HGPs remained significant on multivariate analysis, influencing overall (OS) and metastasis-specific overall survival (MSOS). CD68 + , CD163+ and CD20+ perT infiltrates in UMLM predicted increased OS and MSOS on univariate analysis. Conclusions: TILs and PD-L1 have no predictive value in PUM or UMLM. CD68+ and CD163+TIMs, CD20+ perT lymphocytes, and HGPs are important prognostic factors in UMLMs. [ABSTRACT FROM AUTHOR]