학술논문
The carbohydrate-binding module of Fragaria × ananassa expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities 'in vitro'.
Document Type
Article
Source
Subject
*STRAWBERRIES
*CARBOHYDRATE-binding proteins
*PLANT cell walls
*POLYSACCHARIDES
*ENZYME analysis
*ESCHERICHIA coli
*GENE expression in plants
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Language
ISSN
0918-9440
Abstract
A putative carbohydrate binding module (CBM) from strawberry ( Fragaria × ananassa Duch.) expansin 2 (CBM-FaExp2) was cloned and the encoding protein was over-expressed in Escherichia coli and purified in order to evaluate its capacity to bind different cell wall polysaccharides 'in vitro'. The protein CBM-FaExp2 bound to microcrystalline cellulose, xylan and pectin with different affinities (K = 33.6 ± 0.44 mL g, K = 11.37 ± 0.87 mL g, K = 10.4 ± 0.19 mL g, respectively). According to 'in vitro' enzyme assays, this CBM is able to decrease the activity of cell wall degrading enzymes such as polygalacturonase, endo-glucanase, pectinase and xylanase, probably because the binding of CBM-FaExp2 to the different substrates interferes with enzyme activity. The results suggest that expansins would bind not only cellulose but also a wide range of cell wall polymers. [ABSTRACT FROM AUTHOR]