학술논문
Metabolic Reprogramming in Colon Cancer Cells Persistently Infected with Newcastle Disease Virus.
Document Type
Article
Author
Source
Subject
*LIPID metabolism
*PROTEIN metabolism
*COLON tumors
*DNA
*METABOLIC reprogramming
*DISCRIMINANT analysis
*RAMAN spectroscopy
*CELL survival
*T-test (Statistics)
*CELL proliferation
*DESCRIPTIVE statistics
*RESEARCH funding
*CELL lines
*PARAMYXOVIRUSES
*EPITHELIAL cells
*DATA analysis software
*DRUG resistance in cancer cells
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Language
ISSN
2072-6694
Abstract
Simple Summary: It is vitally important to understand the development of cancer resistance to therapies. Newcastle disease virus (NDV) is a promising oncolytic agent for cancer therapy. A small subpopulation of Caco-2 colon cancer cells persistently infected with NDV was found, which demonstrated resistance to NDV reinfection. By applying Raman spectroscopic and stable isotopic techniques, we found possible mechanisms of the resistant cells to escape from the viral attack, by slowing down their replication and diverting the energy to protein and lipid synthesis. Understanding metabolic reprogramming would be extremely helpful in creating novel cancer treatments to identify and target resistant cells at the single-cell level with great precision. Newcastle disease virus (NDV) is an oncolytic agent against various types of mammalian cancers. As with all cancer therapies, the development of cancer resistance, both innate and acquired, is becoming a challenge. In this study, we investigated persistently NDV-infected Caco-2 colon cancer cells, designated as virus-resistant (VR) Caco-2 cells, which were then able to resist NDV-mediated oncolysis. We applied single-cell Raman spectroscopy, combined with deuterium isotope probing (Raman-DIP) techniques, to investigate the metabolic adaptations and dynamics in VR Caco-2 cells. A linear discriminant analysis (LDA) model demonstrated excellent performance in differentiating VR Caco-2 from Caco-2 cells at single-cell level. By comparing the metabolic profiles in a time-resolved manner, the de novo synthesis of proteins and lipids was found upregulated, along with decreased DNA synthesis in VR Caco-2. The results suggest that VR Caco-2 cells might reprogram their metabolism and divert energy from proliferation to protein synthesis and lipidic modulation. The ability to identify and characterise single resistant cells among a population of cancer cells would help develop a deeper understanding of the resistance mechanisms and better tactics for developing effective cancer treatment. [ABSTRACT FROM AUTHOR]