학술논문

3D imaging and characterization of microlenses and microlens arrays using nonlinear microscopy.
Document Type
Article
Source
Journal of Physics D: Applied Physics. 2013, Vol. 46 Issue 19, p1-9. 9p.
Subject
*THREE-dimensional imaging
*MICROLENSES
*FLUORESCENCE
*EOSIN
*MICROSCOPY
*ELECTRON microscopic diagnosis
Language
ISSN
0022-3727
Abstract
In this work, nonlinear laser scanning microscopy was employed for the characterization and three-dimensional (3D) imaging of microlenses and microlens arrays. Third-harmonic generation and two-photon excitation fluorescence (TPEF) signals were recorded and the obtained data were further processed in order to generate 3D reconstructions of the examined samples. Femtosecond laser pulses (1028 nm) were utilized for excitation. Microlenses were manufactured on Tot'hema and eosin sensitized gelatin layers using a green (532 nm) continuous wave laser beam using the direct laser writing method. The profiles of the microlens surface were obtained from the radial cross-sections, using a triple-Gaussian fit. The analytical shapes of the profiles were also used for ray tracing. Furthermore, the volumes of the microlenses were determined with high precision. The TPEF signal arising from the volume of the material was recorded and the respective 3D spatial fluorescence distribution of the samples was mapped. Nonlinear microscopy modalities have been shown to be a powerful diagnostic tool for microlens characterization as they enable in-depth investigations of the structural properties of the samples, in a nondestructive manner. [ABSTRACT FROM AUTHOR]