학술논문
A Yeast Bioassay for Androgenic and Anti-Androgenic Compounds Based on the NH2 - and COOH-Terminal Interaction of Androgen Recentor.
Document Type
Article
Author
Source
Subject
*BIOLOGICAL assay
*HORMONE receptors
*ANDROGENS
*YEAST
*GREEN fluorescent protein
*PHYSIOLOGICAL effects of resveratrol
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Language
ISSN
0916-8451
Abstract
The article focuses on the application of a yeast bioassay to study the interaction between NH2- and COOH (N–C) terminals of androgen receptors induced by androgens and anti-androgens. The bioassay, accordingly, uses a green fluorescent protein (GFP) as a reporter gene which however produces higher 50% effective concentration (EC50) values of various steroids. It adds the method was also effective in detecting compounds which inhibit the N–C interaction, such as resveratrol.