학술논문
Optimization of an O2 -balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment.
Document Type
Article
Author
Mouré, Anne; Bekir, Sawsen; Bacou, Elodie; Pruvost, Quentin; Haurogné, Karine; Allard, Marie; De Beaurepaire, Laurence; Bosch, Steffi; Riochet, David; Gauthier, Olivier; Blancho, Gilles; Soulillou, Jean-Paul; Poncelet, Denis; Mignot, Grégoire; Courcoux, Philippe; Jegou, Dominique; Bach, Jean-Marie; Mosser, Mathilde
Source
Subject
*TYPE 1 diabetes
*ISLANDS of Langerhans
*EXPERIMENTAL design
*ARTIFICIAL pancreases
*RESPONSE surfaces (Statistics)
*PANCREAS
*ISLANDS
*
*
*
*
*
*
Language
ISSN
2045-2322
Abstract
A bioartificial pancreas (BAP) encapsulating high pancreatic islets concentration is a promising alternative for type 1 diabetes therapy. However, the main limitation of this approach is O2 supply, especially until graft neovascularization. Here, we described a methodology to design an optimal O2-balanced BAP using statistical design of experiment (DoE). A full factorial DoE was first performed to screen two O2-technologies on their ability to preserve pseudo-islet viability and function under hypoxia and normoxia. Then, response surface methodology was used to define the optimal O2-carrier and islet seeding concentrations to maximize the number of viable pseudo-islets in the BAP containing an O2-generator under hypoxia. Monitoring of viability, function and maturation of neonatal pig islets for 15 days in vitro demonstrated the efficiency of the optimal O2-balanced BAP. The findings should allow the design of a more realistic BAP for humans with high islets concentration by maintaining the O2 balance in the device. [ABSTRACT FROM AUTHOR]