부산대학교 도서관

In the last decade, the notion that mRNA modifications are involved in regulation of gene expression was demonstrated in thousands of studies. To date, new technologies and methods allow accurate identification, transcriptome-wide mapping, and functional characterization of a growing number of RNA modifications, providing important insights into the biology of these marks. Most of the methods and approaches were developed for studying m6A, the most prevalent internal mRNA modification. However, unique properties of other RNA modifications stimulated the development of additional approaches. In this technical primer, we will discuss the available tools and approaches for detecting and studying different RNA modifications. A comprehensive overview of the tools to map, manipulate, and study the function of RNA modifications, including m6A and the rest of the growing epitranscriptome. Challenges and limitations are highlighted, with a view toward accelerating our understanding of the function of the diverse chemical modifications of RNA. [ABSTRACT FROM AUTHOR]