부산대학교 도서관

Ethnopharmacological relevance The purple pitcher plant, Sarracenia purpurea , is a medicinal plant used by the Canadian First Nations to treat a wide variety of illnesses. The Mi’kmaq and Wolastoqiyik (Maliseet) peoples of Eastern Canada have traditionally used infusions of S. purpurea for the treatment of tuberculosis-like symptoms. Previous investigations have shown methanolic extracts of S. purpurea to possess antimycobacterial activity. Aim of the study To isolate and identify antimycobacterial constituents from S. purpurea . Materials and Methods Methanolic extracts of S. purpurea were subjected to bioassay guided fractionation using the microplate resazurin assay (MRA) to assess inhibitory activity against Mycobacterium tuberculosis strain H37Ra. The antimycobacterial constituents were identified by NMR, MS and polarimetry. Results The triterpenes betulinaldehyde, β-sitosterol, betulinic acid, and ursolic acid were isolated from S. purpurea . Betulinaldehyde, betulinic acid, and ursolic acid exhibited MICs of 450, 950, and 450 μM and IC 50 s of 98, 169, and 93 μM against M. tuberculosis H37Ra respectively whilst β-sitosterol was inactive (MIC and IC 50 of >1000 μM). Conclusions Betulinaldehyde, betulinic acid, and ursolic acid were identified as the principal constituents responsible for the antimycobacterial activity of S. purpurea . This work is consistent with the ethnopharmacological use of S. purpurea by Canadian First Nations as a treatment against infectious diseases. [ABSTRACT FROM AUTHOR]