학술논문
Determination of the binding properties of p-cresyl glucuronide to human serum albumin.
Document Type
Article
Author
Source
Subject
*GLUCURONIDE synthesis
*SERUM albumin
*FLUORINE compounds
*KIDNEY diseases
*KIDNEY disease treatments
*FUSARIUM toxins
*GENETICS
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Language
ISSN
0300-9084
Abstract
p -Cresyl glucuronide (p-CG) is a by-product of tyrosine metabolism that accumulates in patients with end-stage renal disease. p-CG binding to human serum albumin in physiological conditions (37 °C, pH 7.40) was studied by ultrafiltration (MWCO 10 kDa) and data were analyzed assuming one binding site. The estimated value of the association constant was 2.77 × 10 3 M −1 and a maximal stoichiometry of 3.80 mol per mole. At a concentration relevant for end-stage renal patients, p-CG was 23% bound to albumin. Competition experiments, using fluorescent probes, demonstrated that p-CG did not bind to Sudlow's site I or site II. The p-CG did not interfere with the binding of p-cresyl-sulfate or indoxyl sulfate to serum albumin. [ABSTRACT FROM AUTHOR]