학술논문
Molecular cytogenetic identification of a novel hexaploid wheat-Thinopyrum intermedium partial amphiploid with high protein content.
Document Type
Article
Source
Subject
*PLANT cytogenetics
*WHEAT proteins
*INTROGRESSION (Genetics)
*LEAF rust of wheat
*WHEATGRASS (Wheat)
*PLANT DNA
*FLUORESCENCE in situ hybridization
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Language
ISSN
1300-0152
Abstract
Genomic in situ hybridization and fluorescence in situ hybridization were used to define the cytogenetic constitution of the intergeneric introgression line 29(1-57) obtained from the wheat ? Thinopyrum intermedium (wheatgrass) cross. This line is a highprotein line resistant to leaf rust, yellow rust, and powdery mildew under field conditions and has 42 chromosomes. GISH analysis using a total genomic DNA probe from Thinopyrum intermedium resulted in the identification of 14 chromosomes of wheatgrass origin. The sequential GISH patterns obtained using St-genomic and J-genomic DNA as probes and ABD-genomic DNA from wheat as a blocker led to a better determination of the genomic type of wheatgrass chromosomes. Rearrangements involving Thinopyrum chromosomes and the A, B, and D genomes of wheat were not observed. FISH using the repetitive DNA probes pSc119.2, Afa-family, and pTa71 allowed for identification of all the wheat chromosomes present. [ABSTRACT FROM AUTHOR]