학술논문
BMP2 and BMP4 differentially modulate extracellular matrix-related gene expression in human lung epithelial cells.
Document Type
Article
Source
Subject
*BONE morphogenetic proteins
*GENE expression
*EXTRACELLULAR matrix
*EPITHELIAL cells
*REGENERATION (Biology)
*LUNGS
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Language
ISSN
0892-6638
Abstract
Bone morphogenetic protein 4 (BMP4) is essential for normal lung morphogenesis although the underlying mechanisms are unknown. We are investigating whether BMPs also play a role in remodeling and repair in adult lungs and have examined the effects of BMP4, and its homolog BMP2, on adult lung epithelial cells. When cultured with 100 ng/ml BMP2 or BMP4 for 6 days, human airway BEAS-2B cells exhibited decreased proliferation compared to controls and acquired a mesenchymal-like morphology. Effects were more marked in the presence of BMP4 than BMP2. Since alterations in extracellular matrix (ECM) components occur during lung airway repair and remodeling, and are also observed during epithelial-mesenchymal transition, we examined the mRNA levels of 84 ECM-related genes in 3 independent passages of control, BMP2- and BMP4-treated BEAS-2B cells using real-time PCR arrays (Superarray). BMP4 treatment significantly (p<0.05) upregulated 15 specific ECM-related transcripts including several collagens, metalloproteases and adhesion molecules. No changes occurred in the ECM transcripts surveyed in BMP2-treated cells. Approximately one third of the 84 genes examined were not expressed in BEAS-2B cells. In conclusion, BMP2 and BMP4 differentially modulate ECM-related gene expression in BEAS-2B cells suggesting that BMPs may play a role in regeneration in adult lungs. [ABSTRACT FROM AUTHOR]