학술논문
ENHANCING EFFECT OF HUMAN THYROID STIMULATING HORMONE (hTSH) MONOCLONAL ANTIBODY IN THE hTSH IMMUNORADIOMETRIC ASSAY (IRMA) SYSTEM.
Document Type
Article
Source
Subject
*THYROTROPIN
*MONOCLONAL antibodies
*NUCLEAR activation analysis
*GLYCOPROTEIN hormones
*PITUITARY hormones
*IMMUNOGLOBULINS
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Language
ISSN
1532-1819
Abstract
Different monoclonal antibodies, both commercial and indigenously produced, were evaluated in various combinations to optimize an immunoradiometric assay (IRMA) system for human thyroid stimulating hormone (hTSH). During these studies, it was observed that mixing one of the indigenously produced hTSH monoclonal antibody (2B11) in the hTSH IRMA system using Immunotech (Beckman Coulter, Czech Republic) kit reagents, led to an overall increase in the assay binding and sensitivity (from 0.025 mIU/L to 0.015mIU/L) of this IRMA system. This is not a general property of all monoclonal antibodies against hTSH. The mechanism for this enhancement can be attributed to the formation of a multicomponent complex. [ABSTRACT FROM AUTHOR]