학술논문
Crystallization and preliminary X-ray diffraction analysis of O-acetylserine sulfhydrylase from Aeropyrum pernix K1.
Document Type
Article
Source
Subject
*CRYSTALLIZATION
*CYSTATHIONINE gamma-lyase
*BIOSYNTHESIS
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Language
ISSN
0907-4449
Abstract
Crystals of O-acetylserine sulfhydrylase from Aeropyrum pernix K1 were obtained by the hanging-drop vapour-diffusion method at 298 K. An X-ray diffraction data set was collected to 2.25 Å resolution at 100 K. The crystal belonged to space group P42[sub 1]2, P4[sub 1]2[sub 1]2, P4[sub 2]2[sub 1]2 or P4[sub 3]2[sub 1]2. The unit-cell parameters were a = b = 74.5, c = 276.0 Å. The presence of two subunits of the enzyme per asymmetric unit gives a crystal volume per protein mass (V[sub M]) of 2.28 ų Da[sup -1] and a solvent content of 46%(v/v). [ABSTRACT FROM AUTHOR]