학술논문
Systematic reference sample generation for multiplexed serological assays.
Document Type
Article
Author
Source
Subject
*IMMUNOSPECIFICITY
*QUALITY control
*IMMUNOGLOBULINS
*ANTIGENS
*TUBERCULOSIS complications
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*
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Language
ISSN
2045-2322
Abstract
Quality controls of serological assays have to contain defined amounts of human antibodies specific for the targeted antigen. A prevailing issue for array-based antigen assays is that dozens of antigens are targeted within the same assay. Commonly different patient sera are combined and optimal pools are empirically identified. Here, we report a mathematical approach how an optimal sample pool composition can be systematically calculated and accurately compiled. The approach was used to compose suitable quality controls for a 71 plex Tuberculosis antigen bead array using a limited number of positive human sera. [ABSTRACT FROM AUTHOR]