부산대학교 도서관

Prolonged treatment of human platelets with the adenylate cyclase‐stimulating prostacyclin analog iloprost leads to reduction in cAMP formation. Previous studies have demonstrated that this may be ascribed to modification of both receptor and Gsα function rather than of the catalytic component of adenylate cyclase [Mollner, S., Deppisch, H. & Pfeuffer, T. (1992) Eur. J. Biochem. 210, 539–544]. Iloprost‐induced desensitization was accompanied by the formation of a Gsα‐containing 90‐kDa product in membranes treated with the bifunctional cross‐linker 1,6‐bismaleimidohexane. The cAMP‐inducing prostanoid PGD2, which does not promote desensitization, did not cause formation of the 90‐kDa species either. The long‐term effect of the common G‐protein activator [AlF4]- on human platelet adenylate cyclase was shown in many respects to be comparable with that of iloprost. However, [AlF4]- treatment also failed to induce the 90‐kDa species, showing that different mechanisms of desensitization were operating. Treatment of the cross‐linked 90‐kDa complex with PNGase F demonstrated the glycoprotein nature of the Gsα‐associated component. The 90‐kDa cross‐linked product was purified by consecutive immunoaffinity chromatography and preparative PAGE to apparent homogeneity. Analysis of the purified protein by MS suggested that, besides Gsα, the heavy chain of MHC I (HLA‐A2) was part of the complex. This was confirmed by coprecipitation of Gsα by the monoclonal anti‐(MHC I) antibody W6/32. [ABSTRACT FROM AUTHOR]