학술논문
Yeast RNA-Binding Protein Nab3 Regulates Genes Involved in Nitrogen Metabolism.
Document Type
Article
Author
Source
Subject
*RNA-binding proteins
*GENES
*NITROGEN metabolism
*SACCHAROMYCES cerevisiae
*CATABOLITE repression
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Language
ISSN
0270-7306
Abstract
Termination of Saccharomyces cerevisiae RNA polymerase II (Pol II) transcripts occurs through two alternative pathways. Termination of mRNAs is coupled to cleavage and polyadenylation while noncoding transcripts are terminated through the Nrd1-Nab3-Sen1 (NNS) pathway in a process that is linked to RNA degradation by the nuclear exosome. Some mRNA transcripts are also attenuated through premature termination directed by the NNS complex. In this paper we present the results of nuclear depletion of the NNS component Nab3. As expected, many noncoding RNAs fail to terminate properly. In addition, we observe that nitrogen catabolite-repressed genes are upregulated by Nab3 depletion. [ABSTRACT FROM AUTHOR]