학술논문
Serum complexes of insulin-like growth factor-1 modulate skeletal Integrity and carbohydrate metabolism.
Document Type
Article
Author
Yakax, Shoshana; Rosen, Clifford J.; Bouxsein, Mary L.; Hui Sun; Mejia, Wilson; Kawashima, Yuki; Yingjie Wu; Emerton, Kelly; Williams, Valerie; Jepsen, Karl; Schaffler, Mitchell B.; Majeska, Robert J.; Gavrilova, Oksana; Gutierrez, Mariana; Hwang, David; Permisi, Patricia; Frystyk, Jan; Boisclair, Yves; Pintar, John; Jasper, Héctor
Source
Subject
*SOMATOMEDIN
*INSULIN-like growth factor-binding proteins
*MUSCULOSKELETAL system
*CARBOHYDRATE metabolism
*SOMATOTROPIN
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Language
ISSN
0892-6638
Abstract
Serum insulin-like growth factor (IGF)-1 is secreted mainly by the liver and circulates bound to IGF-binding proteins (IGFBPs), either as binary complexes or ternary complexes with IGFBP-3 or IGFBP-5 and an acid-labile subunit (ALS). The purpose of this study was to genetically dissect the role of IGF-1 circulatory complexes in somatic growth, skeletal integrity, and metabolism. Phenotypic comparisons of controls and four mouse lines with genetic IGF-1 deficits--liver-specific IGF-1 deficiency (LID), ALS knockout (ALSKO), IGFBP-3 (BP3) knockout, and a triply deficient LID/ALSKO/BP3 line--produced several novel findings. 1) All deficient strains had decreased serum IGF-1 levels, but this neither predicted growth potential or skeletal integrity nor defined growth hormone secretion or metabolic abnormalities. 2) IGF-1 deficiency affected development of both cortical and trabecular bone differently, effects apparently dependent on the presence of different circulating IGF-1 complexes. 3) IGFBP-3 deficiency resulted in increased linear growth. In summary, each IGF-1 complex constituent appears to play a distinct role in determining skeletal phenotype, with different effects on cortical and trabecular bone compartments. [ABSTRACT FROM AUTHOR]