학술논문
Studies on Actively Allergized Cells IV. LIGHT AND ELECTRON MICROSCOPIC STUDIES OF THE MORPHOLOGY OF ROSETTE-FORMING CELLS WITH SPECIAL REFERENCE TO PLASMA CELLS.
Document Type
Article
Source
Subject
*PLASMA cells
*LYMPH nodes
*ALLERGIES
*ANTIGENS
*GUINEA pigs as laboratory animals
*LABORATORY mice
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Language
ISSN
0019-2805
Abstract
A proportion of the plasma cells in lymph nodes of allergized guinea-pigs and mice were found to possess membrane-bound receptors for antigen when tested for rosette-formation at 4° by the suspension-centrifugation technique. This was shown by staining rosetted cells with pyronin-methyl green. Rosette-forming cells of the guinea-pig were further examined by staining with fluorescein-conjugated antigen (rabbit Fab') and by electron microscopy. By these techniques it was found that many plasma cells which contain antigen-specific intracellular antibodies do not form rosettes, and that cells of the plasmacytic series represented in the rosetted population are limited to plasmablasts and immature plasma cells. The lack of rosette-forming mature plasma cells suggests that a loss of receptors from the cell-membrane occurs as an accompaniment to maturation. [ABSTRACT FROM AUTHOR]