부산대학교 도서관

Among the wide range of strategiesto target skin repair/regeneration,tissue engineering (TE) with stem cells at the forefront, remainsas the most promising route. Cell sheet (CS) engineering is hereinproposed, taking advantage of particular cell–cell and cell–extracellularmatrix (ECM) interactions and subsequent cellular milieu, to create3D TE constructs to promote full-thickness skin wound regeneration.Human adipose derived stem cells (hASCs) CS were obtained within fivedays using both thermoresponsive and standard cell culture surfaces.hASCs-based constructs were then built by superimposing three CS andtransplanted into full-thickness excisional mice skin wounds withdelayed healing. Constructs obtained using thermoresponsive surfaceswere more stable than the ones from standard cell culture surfacesdue to the natural adhesive character of the respective CS. Both CS-generatingstrategies lead to prolonged hASCs engraftment, although no transdifferentiationphenomena were observed. Moreover, our findings suggest that the transplantedhASCs might be promoting neotissue vascularization and extensivelyinfluencing epidermal morphogenesis, mainly through paracrine actionswith the resident cells. The thicker epidermis, with a higher degreeof maturation characterized by the presence of rete ridges-like structures,as well as a significant number of hair follicles observed after transplantationof the constructs combining the CS obtained from the thermoresponsivesurfaces, reinforced the assumptions of the influence of the transplantedhASCs and the importance of the higher stability of these constructspromoted by cohesive cell–cell and cell–ECM interactions.Overall, this study confirmed the potential of hASCs CS-based constructsto treat full-thickness excisional skin wounds and that their fabricationconditions impact different aspects of skin regeneration, such asneovascularisation, but mainly epidermal morphogenesis. [ABSTRACT FROM AUTHOR]