학술논문
Gene silencing by RNA interference in the house dust mite, Dermatophagoides pteronyssinus.
Document Type
Article
Source
Subject
*DERMATOPHAGOIDES pteronyssinus
*HOUSE dust mites
*GENE silencing
*RNA interference
*GLUTATHIONE transferase
*GENE expression
*INSECTS
*
*
*
*
*
*
Language
ISSN
0890-8508
Abstract
This is the first report of gene silencing by RNA interference (RNAi) in the European house dust mite, Dermatophagoides pteronyssinus , Trouessart, 1897. Using a non-invasive immersion method first developed for the honey bee mite, Varroa destructor , a significant reduction in the expression of D . pteronyssinus glutathione- S -transferase mu-class 1 enzyme ( Dp GST-mu1) was achieved following overnight immersion in double stranded RNA encoding Dp GST-mu1. Although no detrimental phenotypic changes were observed following silencing, this technique can now be used to address fundamental physiological questions and assess the potential therapeutic benefit in silencing D . pteronyssinus target genes in selected domestic situations of high human-mite interface. [ABSTRACT FROM AUTHOR]