학술논문
PCR in lyme neuroborreliosis: a prospective study.
Document Type
Article
Author
Source
Subject
*DIAGNOSTIC use of polymerase chain reaction
*LYME disease
*ETIOLOGY of diseases
*RELAPSING fever
*FEVER
*SPIROCHAETOSIS
*MOLECULAR diagnosis
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Language
ISSN
0001-6314
Abstract
Objectives – DNA proof is the only widely available direct diagnostic tool in Lyme borreliosis. Sensitive PCR detecting of spirochetal DNA was prepared and a prospective study in neuroborreliosis was performed. Materials and methods – 57 hospitalised patients with active neuroborreliosis and proved CSF antibodies synthesis were examined. Nested-PCR (utilizing three targets) was used for the detection of specific DNA in plasma, CSF and urine. Results – Before treatment 36 positive patients (63.1%) were found in all tested specimens in parallel, 28 patients (49.1%) were positive in urine, 20 in CSF (35.0%) and 16 in plasma 28.0%). Later only urine was tested and the following results were obtained: 17 positive patients (30.0%) immediately after treatment, 8 (14.0%) after 3 months and one patient persisted positivity after 6 months. Conclusions – The highest sensitivity of PCR was achieved in the acute period of neuroborreliosis - 63.1% in three body fluids comparing with CSF antibody synthesis. [ABSTRACT FROM AUTHOR]