학술논문
Acyl‐CoA:lysophosphatidylcholine acyltransferase from the unicellular diatom Phaeodactylum tricornutum (PtLPCAT1) is involved in triacylglycerol and galactoglycerolipid synthesis and enhances eicosapentaenoic acid accumulation in recombinant oleaginous yeast
Document Type
Article
Author
Source
Subject
*ACYLTRANSFERASES
*FISH oils
*PHAEODACTYLUM tricornutum
*PHOSPHOCHOLINE
*EICOSAPENTAENOIC acid
*DIATOMS
*UNSATURATED fatty acids
*YEAST
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Language
ISSN
1467-7644
Abstract
Acyl-CoA:lysophosphatidylcholine acyltransferase from the unicellular diatom Phaeodactylum tricornutum (PtLPCAT1) is involved in triacylglycerol and galactoglycerolipid synthesis and enhances eicosapentaenoic acid accumulation in recombinant oleaginous yeast Keywords: CRISPR/Cas9; eicosapentaenoic acid; LPCAT; Phaeodactylum tricornutum; Yarrowia lipolytica EN CRISPR/Cas9 eicosapentaenoic acid LPCAT Phaeodactylum tricornutum Yarrowia lipolytica 238 240 3 01/31/23 20230201 NES 230201 Dietary supplementation of omega-3 very long-chain polyunsaturated fatty acids (VLC-PUFAs), particularly eicosapentaenoic acid (EPA, C20: 5 SP 5,8,11,14,17 sp ) and docosahexaenoic acid (DHA, C22: 6 SP 4,7,10,13,16,19 sp ), is well known to have diverse health benefits. Recently, the biochemical properties of one I P. tricornutum i LPCAT (PtLPCAT1) were reported and it can esterify a 18: 3-, 18: 4- or 20: 4-CoA (n-3) moiety to lysoPC I in vitro i , the later acyl being available for further desaturation to synthesize EPA, contrasting with plant LPCATs (Polo'nska I et al i ., [2]). [Extracted from the article]