학술논문
Retrospective neuropathological diagnosis of TDP‐43 proteinopathies: Factors affecting immunoreactivity of phosphorylated TDP‐43 in fixed post‐mortem brain tissue.
Document Type
Article
Author
Source
Subject
*TDP-43 proteinopathies
*IMMUNOGLOBULINS
*MONOCLONAL antibodies
*COLD storage
*TISSUES
*LIFE sciences
*ALZHEIMER'S disease
*PREFRONTAL cortex
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Language
ISSN
0919-6544
Abstract
This article explores how the immunoreactivity of phosphorylated TDP-43 (pTDP-43) in fixed post-mortem brain tissue can be affected by factors such as long-term storage and prolonged formalin fixation. The study reveals that extended fixation and long-term storage can weaken the immunoreactivity of pTDP-43, but it remains detectable for diagnostic purposes. These findings have implications for the use of brain tissue stored in brain banks for scientific research. The study also found that other proteins, such as amyloid-β and phosphorylated α-synuclein, are not affected by extended fixation or long-term storage, suggesting that existing tissue samples in brain banks can still be used for research, even if they have been stored for many years. [Extracted from the article]