부산대학교 도서관

Aquatic pathogens contribute to the most severe economic loss in fishes. Nanoparticles are being developed as potent antimicrobial agents against various pathogens. The bacteria Aeromonas caviae is one of the most common aquatic pathogens that infects the majority of economically important fishes causing significant losses to the aquaculture industry. This study involved synthesizing and characterizing of a novel chitosan‐selenium nanoflower employing multiple spectroscopic and microscopic approaches. The UV‐vis spectra obtained at 265 nm indicated the formation of the Chitosan‐selenium nanoflower. The particle size analysis revealed the size of the nanoflowers to be 186.3 nm. The transmission electron micrographs revealed a unique nanoflower-like morphology. XRD spectrum revealed amorphous nature. The Raman spectrum showed a strong resonance peak at 254 cm−1 which is a characteristic absorption band for monoclinic Se and α‐Se. Cytotoxicity analysis of the synthesized nanoflowers against isolated fish pathogen Aeromonas caviae showed increasing toxicity in a dose‐dependent manner with maximum cytotoxicity of 75.06% at 1000 µg/mL. The DCFDA assay was conducted to estimate the increase in reactive oxygen species (ROS) production, and the highest percentage increase in ROS of 96.02% was observed at 1000 µg/mL. The lipid peroxidation assay was performed by quantification of the lipid oxidation product malondialdehyde (MDA). The highest percentage of lipid peroxidation was found to occur at a dose of 500 µg/mL. As a result, the synthesized chitosan‐selenium nanoflowers can be exploited as a promising antibacterial treatment against the fish pathogen Aeromonas caviae. [ABSTRACT FROM AUTHOR]