학술논문
Added Value of Next-Generation Sequencing for Multilocus Sequence Typing Analysis of a Pneumocystis jirovecii Pneumonia Outbreak1.
Document Type
Journal Article
Author
Charpentier, Elena; Garnaud, Cécile; Wintenberger, Claire; Bailly, Sébastien; Murat, Jean-Benjamin; Rendu, John; Pavese, Patricia; Drouet, Thibault; Augier, Caroline; Malvezzi, Paolo; Bertrand, Anne Thiébaut; Mallaret, Marie-Reine; Epaulard, Olivier; Cornet, Muriel; Larrat, Sylvie; Maubon, Danièle; Thiébaut-Bertrand, Anne
Source
Subject
*PNEUMOCYSTIS jiroveci
*IMMUNOCOMPROMISED patients
*PNEUMOCYSTIS pneumonia
*NOSOCOMIAL infections
*SPATIOTEMPORAL processes
*FUNGI classification
*COMPARATIVE studies
*DISEASE outbreaks
*BIOLOGICAL evolution
*FUNGI
*GENETIC polymorphisms
*RESEARCH methodology
*MEDICAL cooperation
*RESEARCH
*BIOINFORMATICS
*EVALUATION research
*CASE-control method
*SEQUENCE analysis
*GENOTYPES
*INFECTIOUS disease transmission
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Language
ISSN
1080-6040
Abstract
Pneumocystis jirovecii is a major threat for immunocompromised patients, and clusters of pneumocystis pneumonia (PCP) have been increasingly described in transplant units during the past decade. Exploring an outbreak transmission network requires complementary spatiotemporal and strain-typing approaches. We analyzed a PCP outbreak and demonstrated the added value of next-generation sequencing (NGS) for the multilocus sequence typing (MLST) study of P. jirovecii strains. Thirty-two PCP patients were included. Among the 12 solid organ transplant patients, 5 shared a major and unique genotype that was also found as a minor strain in a sixth patient. A transmission map analysis strengthened the suspicion of nosocomial acquisition of this strain for the 6 patients. NGS-MLST enables accurate determination of subpopulation, which allowed excluding other patients from the transmission network. NGS-MLST genotyping approach was essential to deciphering this outbreak. This innovative approach brings new insights for future epidemiologic studies on this uncultivable opportunistic fungus. [ABSTRACT FROM AUTHOR]