학술논문
Mapping the surface-exposed regions of papaya mosaic virus nanoparticles.
Document Type
Article
Author
Source
Subject
*PAPAYA
*POTEXVIRUSES
*NANOPARTICLES
*PLANT proteins
*CHEMICAL modification of proteins
*SURFACE chemistry
*MOLECULAR self-assembly
*RECOMBINANT proteins
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Language
ISSN
1742-464X
Abstract
In general, the structure of the papaya mosaic virus (PapMV) and other members of the potexviruses is poorly understood. Production of PapMV coat proteins in a bacterial expression system and their self-assembly in vitro into nanoparticles is a very useful tool to study the structure of this virus. Using recombinant PapMV nanoparticles that are similar in shape and appearance to the plant virus, we evaluated surface-exposed regions by two different methods, immunoblot assay and chemical modification with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide or diethyl-pyrocarbonate followed by mass spectrometry. Three regions were targeted by the two techniques. The N- and C-termini were shown to be surfaced exposed as expected. However, the region 125-136 was revealed for the first time as the major surface-exposed region of the nanoparticles. The presence of linear peptides at the surface was finally confirmed using antibodies directed to those peptides. It is likely that region 125-136 plays a key role in the lifecycle of PapMV and other members of the potexvirus group. [ABSTRACT FROM AUTHOR]