학술논문

A linkage map for the hybridising toads Bombina bombina and B. variegata (Anura: Discoglossidae).
Document Type
Article
Source
Heredity. Aug2003, Vol. 91 Issue 2, p136-142. 7p.
Subject
*HYBRID zones
*BOMBINA bombina
*MICROSATELLITE repeats
*ISOENZYMES
Language
ISSN
0018-067X
Abstract
Stable hybrid zones in which ecologically divergent taxa give rise to a range of recombinants are natural laboratories in which the genetic basis of adaptation and reproductive isolation can be unraveled. One such hybrid zone is formed by the fire-bellied toads Bombina bombina and B. variegata (Anura: Discoglossidae). Adaptations to permanent and ephemeral breeding habitats, respectively, have shaped numerous phenotypic differences between the taxa. All of these are, in principle, candidates for a genetic dissection via QTL mapping. We present here a linkage map of 28 codominant and 10 dominant markers in the Bombina genome. In an F2 cross, markers that were mainly microsatellites, SSCPs or allozymes were mapped to 20 linkage groups. Among the 40 isolated CA microsatellites, we noted a preponderance of compound and frequently interleaved CA-TA repeats as well as a striking polarity at the 5' end of the repeats. [ABSTRACT FROM AUTHOR]