학술논문
Precision Tracing of Household Dengue Spread Using Inter- and Intra-Host Viral Variation Data, Kamphaeng Phet, Thailand.
Document Type
Journal Article
Author
Berry, Irina Maljkovic; Melendrez, Melanie C.; Pollett, Simon; Figueroa, Katherine; Buddhari, Darunee; Klungthong, Chonticha; Nisalak, Ananda; Panciera, Michael; Thaisomboonsuk, Butsaya; Tao Li; Vallard, Tyghe G.; Macareo, Louis; In-Kyu Yoon; Thomas, Stephen J.; Endy, Timothy; Jarman, Richard G.; Li, Tao; Yoon, In-Kyu
Source
Subject
*VIRAL variation
*DENGUE
*HOUSEHOLDS
*VIRAL transmission
*RESEARCH
*FLAVIVIRUSES
*RESEARCH methodology
*MEDICAL cooperation
*EVALUATION research
*COMPARATIVE studies
*RESEARCH funding
*LONGITUDINAL method
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Language
ISSN
1080-6040
Abstract
Dengue control approaches are best informed by granular spatial epidemiology of these viruses, yet reconstruction of inter- and intra-household transmissions is limited when analyzing case count, serologic, or genomic consensus sequence data. To determine viral spread on a finer spatial scale, we extended phylogenomic discrete trait analyses to reconstructions of house-to-house transmissions within a prospective cluster study in Kamphaeng Phet, Thailand. For additional resolution and transmission confirmation, we mapped dengue intra-host single nucleotide variants on the taxa of these time-scaled phylogenies. This approach confirmed 19 household transmissions and revealed that dengue disperses an average of 70 m per day between households in these communities. We describe an evolutionary biology framework for the resolution of dengue transmissions that cannot be differentiated based on epidemiologic and consensus genome data alone. This framework can be used as a public health tool to inform control approaches and enable precise tracing of dengue transmissions. [ABSTRACT FROM AUTHOR]