학술논문
A novel method for detecting antigen-specific human regulatory T cells
Document Type
Article
Source
Subject
*EPITOPES
*T cells
*PEPTIDES
*LYMPHOCYTES
*TUMOR markers
*TUMOR antigens
*IMMUNE recognition
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Language
ISSN
0022-1759
Abstract
Abstract: Antigenic epitopes recognized by FoxP3+ regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognition. Culture conditions and re-stimulation time have been optimized, allowing the detection of even very rare Treg, such as those specific to tumor antigens. [Copyright &y& Elsevier]