학술논문

DNA barcodes as a tool in biodiversity research: testing pre-existing taxonomic hypotheses in Delphic Apollo butterflies (Lepidoptera, Papilionidae).
Document Type
Article
Source
Systematics & Biodiversity. Dec2016, Vol. 14 Issue 6, p599-613. 15p.
Subject
*GENETIC barcoding
*BIODIVERSITY research
*BUTTERFLIES
*CLASSIFICATION of insects
*LEPIDOPTERA
*PAPILIONIDAE
Language
ISSN
1477-2000
Abstract
Numerous studies have demonstrated that DNA barcoding is an effective tool for detecting DNA clusters, which can be viewed as operational taxonomic units (OTUs), useful for biodiversity research. Frequently, the OTUs in these studies remained unnamed, not connected with pre-existing taxonomic hypotheses, and thus did not really contribute to feasible estimation of species number and adjustment of species boundaries. For the majority of organisms, taxonomy is very complicated with numerous, often contradictory interpretations of the same characters, which may result in several competing checklists using different specific and subspecific names to describe the same sets of populations. The highly species-rich genusParnassius(Lepidoptera: Papilionidae) is but one example, such as several mutually exclusive taxonomic systems have been suggested to describe the phenotypic diversity found among its populations. Here we provide an explicit flow chart describing how the DNA barcodes can be combined with the existing knowledge of morphology-based taxonomy and geography (sympatry versus allopatry) of the studied populations in order to support, reject or modify the pre-existing taxonomic hypotheses. We then apply this flow chart to reorganize the taxa within theParnassius delphiusspecies group, solving long-standing taxonomic problems. [ABSTRACT FROM AUTHOR]