부산대학교 도서관

Context:SeveralPolygonumspecies (Polygonaceae) are used in traditional medicine in Asia, Europe and Africa to treat inflammation and diabetes. Objective:Evaluate thein vitroantioxidant, anti-inflammatory and antidiabetic potential of methanol and dichloromethane extracts of leaves and roots of the halophytePolygonum maritimumL. Material and methods:Antioxidant activity was determined (up to 1 mg/mL) as radical-scavenging activity (RSA) of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), copper (CCA) and iron (ICA) chelating activities and iron reducing power (FRAP). NO production was measured in lipopolysaccharide (LPS)-stimulated macrophages for 24 h at concentrations up to 100 μg/mL and antidiabetic potential was assessed by α-amylase and α-glucosidase inhibition (up to 10 mg/mL) assays. The phytochemical composition of the extracts was determined by gas chromatography-mass spectrometry (GC-MS). Results:The methanol leaf extract had the highest activity against DPPH•(IC50= 26 μg/mL) and ABTS+•(IC50= 140 μg/mL), FRAP (IC50= 48 μg/mL) and CCA (IC50= 770 μg/mL). Only the dichloromethane leaf extract (LDCM) showed anti-inflammatory activity (IC50= 48 μg/mL). The methanol root (IC50= 19 μg/mL) and leaf (IC50= 29 μg/mL) extracts strongly inhibited baker’s yeast α-glucosidase, but LDCM had higher rat’s α-glucosidase inhibition (IC50= 2527 μg/mL) than acarbose (IC50= 4638 μg/mL). GC-MS analysis identified β-sitosterol, stigmasterol, 1-octacosanol and linolenic acid as possible molecules responsible for the observed bioactivities. Conclusions:Our findings suggestP. maritimumas a source of high-value health promoting commodities for alleviating symptoms associated with oxidative and inflammatory diseases, including diabetes. [ABSTRACT FROM PUBLISHER]