학술논문
Virtual screening for novel quorum sensing inhibitors to eradicate biofilm formation of Pseudomonas aeruginosa.
Document Type
Article
Author
Source
Subject
*QUORUM sensing
*MICROBIAL genetics
*CELL communication
*BIOFILMS
*PSEUDOMONAS aeruginosa
*ANTIBACTERIAL agents
*PROTEOLYSIS
*PROTEIN metabolism
*PROTEOLYTIC enzymes
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Language
ISSN
0175-7598
Abstract
The automated docking program DOCK 5.3.0 was applied to screening for quorum sensing inhibitors (QSIs) of Peudomonus aeruginosa from a database containing 51 active components of Traditional Chinese Medicines with antibacterial activity. Five potential QSIs were revealed by the computer-based virtual screening. The compounds 3, 4, 5, 6, 7 inhibit biofilm formation of P. aeruginosa at a concentration of 200 μM. Compound 4 (baicalein) does not inhibit the growth of P. aeruginosa; however, it significantly inhibits biofilm formation of the bacteria at a lower concentration of 20 μM and promoted proteolysis of the signal receptor TraR protein in Escherichia coli at 4–40 mM. Baicalein and ampicillin showed synergistic activity against P. aeruginosa. These results suggested that baicalein can interfere with quorum sensing system of P. aeruginosa and will be developed as antibacterial agent with novel target. [ABSTRACT FROM AUTHOR]