학술논문
Deletion of NTH1 and HSP12 increases the freeze–thaw resistance of baker's yeast in bread dough.
Document Type
Article
Author
Source
Subject
*SACCHAROMYCES cerevisiae
*HEAT shock proteins
*DOUGH
*GENOME editing
*BAKING industry
*CRYOPRESERVATION of cells
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Language
ISSN
1475-2859
Abstract
Background: The intracellular molecule trehalose in Saccharomyces cerevisiae may have a major protective function under extreme environmental conditions. NTH1 is one gene which expresses trehalase to degrade trehalose. Small heat shock protein 12 (HSP12 expressed) plays a role in protecting membranes and enhancing freezing stress tolerance. Results: An optimized S. cerevisiae CRISPR-Cpf1 genome-editing system was constructed. Multiplex genome editing using a single crRNA array was shown to be functional. NTH1 or/and HSP12 knockout in S. cerevisiae enhanced the freezing stress tolerance and improved the leavening ability after freezing and thawing. Conclusions: Deleting NTH1 in the combination with deleting HSP12 would strengthen the freezing tolerance and protect the cell viability from high rates of death in longer-term freezing. It provides valuable insights for breeding novel S. cerevisiae strains for the baking industry through a more precise, speedy, and economic genome-editing system. [ABSTRACT FROM AUTHOR]