학술논문
Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants.
Document Type
Journal Article
Author
Lin, Gu-Lung; Golubchik, Tanya; Drysdale, Simon; O'Connor, Daniel; Jefferies, Kimberley; Brown, Anthony; Cesare, Mariateresa de; Bonsall, David; Ansari, M Azim; Aerssens, Jeroen; Bont, Louis; Openshaw, Peter; Martinón-Torres, Federico; Bowden, Rory; Pollard, Andrew J; Investigators, RESCEU; de Cesare, Mariateresa; RESCEU Investigators (CORPORATE AUTHOR)
Source
Subject
*RESPIRATORY syncytial virus infections
*VIRAL genetics
*CLINICAL trial registries
*MEDICAL genetics
*RESPIRATORY syncytial virus
*RESEARCH
*SEQUENCE analysis
*BIOLOGICAL evolution
*RESEARCH methodology
*MEDICAL cooperation
*EVALUATION research
*GENE expression
*COMPARATIVE studies
*GENOMES
*GENE expression profiling
*RESEARCH funding
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Language
ISSN
0022-1899
Abstract
Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between 2 RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development. Clinical Trials Registration. NCT03627572 and NCT03756766. [ABSTRACT FROM AUTHOR]