학술논문
Introduction of an electrochemical point‐of‐care assay for quantitative determination of paracetamol in finger‐prick capillary whole blood samples.
Document Type
Article
Author
Source
Subject
*LIQUID chromatography-mass spectrometry
*ACETAMINOPHEN
*HIGH performance liquid chromatography
*BLOOD sampling
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Language
ISSN
0306-5251
Abstract
Aims: Measuring venous plasma paracetamol concentrations is time‐ and resource‐consuming. We aimed to validate a novel electrochemical point‐of‐care (POC) assay for rapid paracetamol concentration determinations. Methods: Twelve healthy volunteers received 1 g oral paracetamol, and its concentrations were analysed 10 times over 12 h for capillary whole blood (POC), venous plasma (high‐performance liquid chromatography tandem mass spectrometry (HPLC‐MS/MS)), and dried capillary blood (HPLC‐MS/MS). Results: At concentrations >30 μM, POC showed upward biases of 20% (95% limits of agreement [LOA] −22 to 62) and 7% (95% LOA −23 to 38) compared with venous plasma and capillary blood HPLC‐MS/MS, respectively. There were no significant differences between mean concentrations for the paracetamol elimination phase. Conclusions: Upward biases in POC compared with venous plasma HPLC‐MS/MS were likely due to higher paracetamol concentrations in capillary blood than in venous plasma and to faulty individual sensors. The novel POC method is a promising tool for paracetamol concentration analysis. [ABSTRACT FROM AUTHOR]